jak2 sc-278 antibody Search Results


jak2 antibody sc 278  (Santa Cruz Biotechnology)
96
Santa Cruz Biotechnology jak2 antibody sc 278
ARMS and α-syntrophin enhanced the EphA4-induced Jak/Stat activation. (A) COS7 cells were transfected with EphA4, EphA4 KD mutant, or ARMS, and cell lysate was immunoblotted with antibodies against phosphorylated Tyr 1007/1008 of mouse <t>Jak2,</t> Tyr 1054/1055 of human Tyk2, Tyr 701 of Stat1, and Thr 202 /Tyr 204 of ERK1/2. p-Tyr; phosphorylated Tyr. (B) Quantification of the Western blots. y axis represents the fold change of tyrosine phosphorylation. n = 3; *, P < 0.05; **, P < 0.005. (C) COS7 cells were transfected with EphA4, ARMS, α-syntrophin, or α-syntrophin mPDZ, and cell lysate was immunoblotted with antibodies against phosphorylated Tyr 1007/1008 of mouse Jak2, Tyr 1054/1055 of human Tyk2, Tyr 701 of Stat1, and Thr 202 /Tyr 204 of ERK1/2. (D) Quantification of the Western blots. n = 3; *, P < 0.05; **, P < 0.005. Error bars represent the SEM.
Jak2 Antibody Sc 278, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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primary antibody against jak2 sc-278  (Santa Cruz Biotechnology)
90
Santa Cruz Biotechnology primary antibody against jak2 sc-278
ARMS and α-syntrophin enhanced the EphA4-induced Jak/Stat activation. (A) COS7 cells were transfected with EphA4, EphA4 KD mutant, or ARMS, and cell lysate was immunoblotted with antibodies against phosphorylated Tyr 1007/1008 of mouse <t>Jak2,</t> Tyr 1054/1055 of human Tyk2, Tyr 701 of Stat1, and Thr 202 /Tyr 204 of ERK1/2. p-Tyr; phosphorylated Tyr. (B) Quantification of the Western blots. y axis represents the fold change of tyrosine phosphorylation. n = 3; *, P < 0.05; **, P < 0.005. (C) COS7 cells were transfected with EphA4, ARMS, α-syntrophin, or α-syntrophin mPDZ, and cell lysate was immunoblotted with antibodies against phosphorylated Tyr 1007/1008 of mouse Jak2, Tyr 1054/1055 of human Tyk2, Tyr 701 of Stat1, and Thr 202 /Tyr 204 of ERK1/2. (D) Quantification of the Western blots. n = 3; *, P < 0.05; **, P < 0.005. Error bars represent the SEM.
Primary Antibody Against Jak2 Sc 278, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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jak2 hr 758 antibody  (Santa Cruz Biotechnology)
96
Santa Cruz Biotechnology jak2 hr 758 antibody
Effects of acute exercise on metabolic parameters and TUB regulation. (A) Body weight (g); (B) 24 h of food intake (g); (C) serum IL-6 levels (pg/ml); and (D) Western blot from hypothalamic lysates showing IL-6 expression in C57BL/6J mice (12–15 weeks of age) under resting conditions (Sed) or after swimming exercise (Exe). B6- tub/+ or B6- tub/tub mice (6–8 weeks of age) were divided into the following groups: B6- tub/+ : sedentary plus vehicle; sedentary plus IL-6 ICV; exercise plus vehicle; exercise plus ABIL6 (IL-6 antibody); B6- tub/tub : exercise plus vehicle. (E) Evaluation of the cumulative food intake (g) at 4 h, 12 h, and 24 h; (H) AgRP and (I) POMC mRNA expression in the arcuate nucleus of the hypothalamus. Western blot showing (F) TUB tyrosine phosphorylation and (G) TUB associated with <t>JAK2</t> from hypothalamus lysates. The exercise was swimming. All mice were male. ICV: intracerebroventricular. All values are expressed as means ± standard deviation (SD). (A–C) : Sed ( n = 7) and Exe ( n = 6). (D,F,G) : ( n = 4 each group). (E,H,I) : ( n = 5 each group). Unpaired two-tailed t -tests were used to analyze (A–D) ; two-way ANOVA was used to analyze (E) ; one-way ANOVA with Tukey’s multiple comparisons tests was used to analyze (F–I) . * p < 0.0001 vs. other groups; & p < 0.05 vs. other groups; # [Sed + IL-6 vs. Sed + Veh ( p = 0.0411); Sed + Veh vs. Exe + Veh ( p = 0.0002); Sed + IL-6 vs. Exe (B6- tub/tub ) ( p = 0.0314); Exe + Veh vs. Exe (ABIL6) ( p = 0.0004); Exe + Veh vs. Exe (B6- tub/tub ) ( p < 0.0001)].
Jak2 Hr 758 Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti jak2  (Santa Cruz Biotechnology)
96
Santa Cruz Biotechnology anti jak2
Effects of acute exercise on metabolic parameters and TUB regulation. (A) Body weight (g); (B) 24 h of food intake (g); (C) serum IL-6 levels (pg/ml); and (D) Western blot from hypothalamic lysates showing IL-6 expression in C57BL/6J mice (12–15 weeks of age) under resting conditions (Sed) or after swimming exercise (Exe). B6- tub/+ or B6- tub/tub mice (6–8 weeks of age) were divided into the following groups: B6- tub/+ : sedentary plus vehicle; sedentary plus IL-6 ICV; exercise plus vehicle; exercise plus ABIL6 (IL-6 antibody); B6- tub/tub : exercise plus vehicle. (E) Evaluation of the cumulative food intake (g) at 4 h, 12 h, and 24 h; (H) AgRP and (I) POMC mRNA expression in the arcuate nucleus of the hypothalamus. Western blot showing (F) TUB tyrosine phosphorylation and (G) TUB associated with <t>JAK2</t> from hypothalamus lysates. The exercise was swimming. All mice were male. ICV: intracerebroventricular. All values are expressed as means ± standard deviation (SD). (A–C) : Sed ( n = 7) and Exe ( n = 6). (D,F,G) : ( n = 4 each group). (E,H,I) : ( n = 5 each group). Unpaired two-tailed t -tests were used to analyze (A–D) ; two-way ANOVA was used to analyze (E) ; one-way ANOVA with Tukey’s multiple comparisons tests was used to analyze (F–I) . * p < 0.0001 vs. other groups; & p < 0.05 vs. other groups; # [Sed + IL-6 vs. Sed + Veh ( p = 0.0411); Sed + Veh vs. Exe + Veh ( p = 0.0002); Sed + IL-6 vs. Exe (B6- tub/tub ) ( p = 0.0314); Exe + Veh vs. Exe (ABIL6) ( p = 0.0004); Exe + Veh vs. Exe (B6- tub/tub ) ( p < 0.0001)].
Anti Jak2, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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irs 1 sc 559 obr  (Santa Cruz Biotechnology)
96
Santa Cruz Biotechnology irs 1 sc 559 obr
Effects of acute exercise on metabolic parameters and TUB regulation. (A) Body weight (g); (B) 24 h of food intake (g); (C) serum IL-6 levels (pg/ml); and (D) Western blot from hypothalamic lysates showing IL-6 expression in C57BL/6J mice (12–15 weeks of age) under resting conditions (Sed) or after swimming exercise (Exe). B6- tub/+ or B6- tub/tub mice (6–8 weeks of age) were divided into the following groups: B6- tub/+ : sedentary plus vehicle; sedentary plus IL-6 ICV; exercise plus vehicle; exercise plus ABIL6 (IL-6 antibody); B6- tub/tub : exercise plus vehicle. (E) Evaluation of the cumulative food intake (g) at 4 h, 12 h, and 24 h; (H) AgRP and (I) POMC mRNA expression in the arcuate nucleus of the hypothalamus. Western blot showing (F) TUB tyrosine phosphorylation and (G) TUB associated with <t>JAK2</t> from hypothalamus lysates. The exercise was swimming. All mice were male. ICV: intracerebroventricular. All values are expressed as means ± standard deviation (SD). (A–C) : Sed ( n = 7) and Exe ( n = 6). (D,F,G) : ( n = 4 each group). (E,H,I) : ( n = 5 each group). Unpaired two-tailed t -tests were used to analyze (A–D) ; two-way ANOVA was used to analyze (E) ; one-way ANOVA with Tukey’s multiple comparisons tests was used to analyze (F–I) . * p < 0.0001 vs. other groups; & p < 0.05 vs. other groups; # [Sed + IL-6 vs. Sed + Veh ( p = 0.0411); Sed + Veh vs. Exe + Veh ( p = 0.0002); Sed + IL-6 vs. Exe (B6- tub/tub ) ( p = 0.0314); Exe + Veh vs. Exe (ABIL6) ( p = 0.0004); Exe + Veh vs. Exe (B6- tub/tub ) ( p < 0.0001)].
Irs 1 Sc 559 Obr, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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tyk2 antibody  (Cell Signaling Technology Inc)
90
Cell Signaling Technology Inc tyk2 antibody
Effects of acute exercise on metabolic parameters and TUB regulation. (A) Body weight (g); (B) 24 h of food intake (g); (C) serum IL-6 levels (pg/ml); and (D) Western blot from hypothalamic lysates showing IL-6 expression in C57BL/6J mice (12–15 weeks of age) under resting conditions (Sed) or after swimming exercise (Exe). B6- tub/+ or B6- tub/tub mice (6–8 weeks of age) were divided into the following groups: B6- tub/+ : sedentary plus vehicle; sedentary plus IL-6 ICV; exercise plus vehicle; exercise plus ABIL6 (IL-6 antibody); B6- tub/tub : exercise plus vehicle. (E) Evaluation of the cumulative food intake (g) at 4 h, 12 h, and 24 h; (H) AgRP and (I) POMC mRNA expression in the arcuate nucleus of the hypothalamus. Western blot showing (F) TUB tyrosine phosphorylation and (G) TUB associated with <t>JAK2</t> from hypothalamus lysates. The exercise was swimming. All mice were male. ICV: intracerebroventricular. All values are expressed as means ± standard deviation (SD). (A–C) : Sed ( n = 7) and Exe ( n = 6). (D,F,G) : ( n = 4 each group). (E,H,I) : ( n = 5 each group). Unpaired two-tailed t -tests were used to analyze (A–D) ; two-way ANOVA was used to analyze (E) ; one-way ANOVA with Tukey’s multiple comparisons tests was used to analyze (F–I) . * p < 0.0001 vs. other groups; & p < 0.05 vs. other groups; # [Sed + IL-6 vs. Sed + Veh ( p = 0.0411); Sed + Veh vs. Exe + Veh ( p = 0.0002); Sed + IL-6 vs. Exe (B6- tub/tub ) ( p = 0.0314); Exe + Veh vs. Exe (ABIL6) ( p = 0.0004); Exe + Veh vs. Exe (B6- tub/tub ) ( p < 0.0001)].
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stat5 sc 835 antibodies  (Santa Cruz Biotechnology)
96
Santa Cruz Biotechnology stat5 sc 835 antibodies
Effects of acute exercise on metabolic parameters and TUB regulation. (A) Body weight (g); (B) 24 h of food intake (g); (C) serum IL-6 levels (pg/ml); and (D) Western blot from hypothalamic lysates showing IL-6 expression in C57BL/6J mice (12–15 weeks of age) under resting conditions (Sed) or after swimming exercise (Exe). B6- tub/+ or B6- tub/tub mice (6–8 weeks of age) were divided into the following groups: B6- tub/+ : sedentary plus vehicle; sedentary plus IL-6 ICV; exercise plus vehicle; exercise plus ABIL6 (IL-6 antibody); B6- tub/tub : exercise plus vehicle. (E) Evaluation of the cumulative food intake (g) at 4 h, 12 h, and 24 h; (H) AgRP and (I) POMC mRNA expression in the arcuate nucleus of the hypothalamus. Western blot showing (F) TUB tyrosine phosphorylation and (G) TUB associated with <t>JAK2</t> from hypothalamus lysates. The exercise was swimming. All mice were male. ICV: intracerebroventricular. All values are expressed as means ± standard deviation (SD). (A–C) : Sed ( n = 7) and Exe ( n = 6). (D,F,G) : ( n = 4 each group). (E,H,I) : ( n = 5 each group). Unpaired two-tailed t -tests were used to analyze (A–D) ; two-way ANOVA was used to analyze (E) ; one-way ANOVA with Tukey’s multiple comparisons tests was used to analyze (F–I) . * p < 0.0001 vs. other groups; & p < 0.05 vs. other groups; # [Sed + IL-6 vs. Sed + Veh ( p = 0.0411); Sed + Veh vs. Exe + Veh ( p = 0.0002); Sed + IL-6 vs. Exe (B6- tub/tub ) ( p = 0.0314); Exe + Veh vs. Exe (ABIL6) ( p = 0.0004); Exe + Veh vs. Exe (B6- tub/tub ) ( p < 0.0001)].
Stat5 Sc 835 Antibodies, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti tyk2  (Santa Cruz Biotechnology)
92
Santa Cruz Biotechnology anti tyk2
Effects of acute exercise on metabolic parameters and TUB regulation. (A) Body weight (g); (B) 24 h of food intake (g); (C) serum IL-6 levels (pg/ml); and (D) Western blot from hypothalamic lysates showing IL-6 expression in C57BL/6J mice (12–15 weeks of age) under resting conditions (Sed) or after swimming exercise (Exe). B6- tub/+ or B6- tub/tub mice (6–8 weeks of age) were divided into the following groups: B6- tub/+ : sedentary plus vehicle; sedentary plus IL-6 ICV; exercise plus vehicle; exercise plus ABIL6 (IL-6 antibody); B6- tub/tub : exercise plus vehicle. (E) Evaluation of the cumulative food intake (g) at 4 h, 12 h, and 24 h; (H) AgRP and (I) POMC mRNA expression in the arcuate nucleus of the hypothalamus. Western blot showing (F) TUB tyrosine phosphorylation and (G) TUB associated with <t>JAK2</t> from hypothalamus lysates. The exercise was swimming. All mice were male. ICV: intracerebroventricular. All values are expressed as means ± standard deviation (SD). (A–C) : Sed ( n = 7) and Exe ( n = 6). (D,F,G) : ( n = 4 each group). (E,H,I) : ( n = 5 each group). Unpaired two-tailed t -tests were used to analyze (A–D) ; two-way ANOVA was used to analyze (E) ; one-way ANOVA with Tukey’s multiple comparisons tests was used to analyze (F–I) . * p < 0.0001 vs. other groups; & p < 0.05 vs. other groups; # [Sed + IL-6 vs. Sed + Veh ( p = 0.0411); Sed + Veh vs. Exe + Veh ( p = 0.0002); Sed + IL-6 vs. Exe (B6- tub/tub ) ( p = 0.0314); Exe + Veh vs. Exe (ABIL6) ( p = 0.0004); Exe + Veh vs. Exe (B6- tub/tub ) ( p < 0.0001)].
Anti Tyk2, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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phospho-jak2-specific rabbit polyclonal antiserum  (Biosource Technologies Inc)
90
Biosource Technologies Inc phospho-jak2-specific rabbit polyclonal antiserum
Effects of acute exercise on metabolic parameters and TUB regulation. (A) Body weight (g); (B) 24 h of food intake (g); (C) serum IL-6 levels (pg/ml); and (D) Western blot from hypothalamic lysates showing IL-6 expression in C57BL/6J mice (12–15 weeks of age) under resting conditions (Sed) or after swimming exercise (Exe). B6- tub/+ or B6- tub/tub mice (6–8 weeks of age) were divided into the following groups: B6- tub/+ : sedentary plus vehicle; sedentary plus IL-6 ICV; exercise plus vehicle; exercise plus ABIL6 (IL-6 antibody); B6- tub/tub : exercise plus vehicle. (E) Evaluation of the cumulative food intake (g) at 4 h, 12 h, and 24 h; (H) AgRP and (I) POMC mRNA expression in the arcuate nucleus of the hypothalamus. Western blot showing (F) TUB tyrosine phosphorylation and (G) TUB associated with <t>JAK2</t> from hypothalamus lysates. The exercise was swimming. All mice were male. ICV: intracerebroventricular. All values are expressed as means ± standard deviation (SD). (A–C) : Sed ( n = 7) and Exe ( n = 6). (D,F,G) : ( n = 4 each group). (E,H,I) : ( n = 5 each group). Unpaired two-tailed t -tests were used to analyze (A–D) ; two-way ANOVA was used to analyze (E) ; one-way ANOVA with Tukey’s multiple comparisons tests was used to analyze (F–I) . * p < 0.0001 vs. other groups; & p < 0.05 vs. other groups; # [Sed + IL-6 vs. Sed + Veh ( p = 0.0411); Sed + Veh vs. Exe + Veh ( p = 0.0002); Sed + IL-6 vs. Exe (B6- tub/tub ) ( p = 0.0314); Exe + Veh vs. Exe (ABIL6) ( p = 0.0004); Exe + Veh vs. Exe (B6- tub/tub ) ( p < 0.0001)].
Phospho Jak2 Specific Rabbit Polyclonal Antiserum, supplied by Biosource Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti-src gd11 mouse antibody  (Millipore)
90
Millipore anti-src gd11 mouse antibody
Effects of acute exercise on metabolic parameters and TUB regulation. (A) Body weight (g); (B) 24 h of food intake (g); (C) serum IL-6 levels (pg/ml); and (D) Western blot from hypothalamic lysates showing IL-6 expression in C57BL/6J mice (12–15 weeks of age) under resting conditions (Sed) or after swimming exercise (Exe). B6- tub/+ or B6- tub/tub mice (6–8 weeks of age) were divided into the following groups: B6- tub/+ : sedentary plus vehicle; sedentary plus IL-6 ICV; exercise plus vehicle; exercise plus ABIL6 (IL-6 antibody); B6- tub/tub : exercise plus vehicle. (E) Evaluation of the cumulative food intake (g) at 4 h, 12 h, and 24 h; (H) AgRP and (I) POMC mRNA expression in the arcuate nucleus of the hypothalamus. Western blot showing (F) TUB tyrosine phosphorylation and (G) TUB associated with <t>JAK2</t> from hypothalamus lysates. The exercise was swimming. All mice were male. ICV: intracerebroventricular. All values are expressed as means ± standard deviation (SD). (A–C) : Sed ( n = 7) and Exe ( n = 6). (D,F,G) : ( n = 4 each group). (E,H,I) : ( n = 5 each group). Unpaired two-tailed t -tests were used to analyze (A–D) ; two-way ANOVA was used to analyze (E) ; one-way ANOVA with Tukey’s multiple comparisons tests was used to analyze (F–I) . * p < 0.0001 vs. other groups; & p < 0.05 vs. other groups; # [Sed + IL-6 vs. Sed + Veh ( p = 0.0411); Sed + Veh vs. Exe + Veh ( p = 0.0002); Sed + IL-6 vs. Exe (B6- tub/tub ) ( p = 0.0314); Exe + Veh vs. Exe (ABIL6) ( p = 0.0004); Exe + Veh vs. Exe (B6- tub/tub ) ( p < 0.0001)].
Anti Src Gd11 Mouse Antibody, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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antiphospho jak2 t221  (Proteintech)
96
Proteintech antiphospho jak2 t221
Effects of acute exercise on metabolic parameters and TUB regulation. (A) Body weight (g); (B) 24 h of food intake (g); (C) serum IL-6 levels (pg/ml); and (D) Western blot from hypothalamic lysates showing IL-6 expression in C57BL/6J mice (12–15 weeks of age) under resting conditions (Sed) or after swimming exercise (Exe). B6- tub/+ or B6- tub/tub mice (6–8 weeks of age) were divided into the following groups: B6- tub/+ : sedentary plus vehicle; sedentary plus IL-6 ICV; exercise plus vehicle; exercise plus ABIL6 (IL-6 antibody); B6- tub/tub : exercise plus vehicle. (E) Evaluation of the cumulative food intake (g) at 4 h, 12 h, and 24 h; (H) AgRP and (I) POMC mRNA expression in the arcuate nucleus of the hypothalamus. Western blot showing (F) TUB tyrosine phosphorylation and (G) TUB associated with <t>JAK2</t> from hypothalamus lysates. The exercise was swimming. All mice were male. ICV: intracerebroventricular. All values are expressed as means ± standard deviation (SD). (A–C) : Sed ( n = 7) and Exe ( n = 6). (D,F,G) : ( n = 4 each group). (E,H,I) : ( n = 5 each group). Unpaired two-tailed t -tests were used to analyze (A–D) ; two-way ANOVA was used to analyze (E) ; one-way ANOVA with Tukey’s multiple comparisons tests was used to analyze (F–I) . * p < 0.0001 vs. other groups; & p < 0.05 vs. other groups; # [Sed + IL-6 vs. Sed + Veh ( p = 0.0411); Sed + Veh vs. Exe + Veh ( p = 0.0002); Sed + IL-6 vs. Exe (B6- tub/tub ) ( p = 0.0314); Exe + Veh vs. Exe (ABIL6) ( p = 0.0004); Exe + Veh vs. Exe (B6- tub/tub ) ( p < 0.0001)].
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irs 1  (Santa Cruz Biotechnology)
96
Santa Cruz Biotechnology irs 1
Effects of acute exercise on metabolic parameters and TUB regulation. (A) Body weight (g); (B) 24 h of food intake (g); (C) serum IL-6 levels (pg/ml); and (D) Western blot from hypothalamic lysates showing IL-6 expression in C57BL/6J mice (12–15 weeks of age) under resting conditions (Sed) or after swimming exercise (Exe). B6- tub/+ or B6- tub/tub mice (6–8 weeks of age) were divided into the following groups: B6- tub/+ : sedentary plus vehicle; sedentary plus IL-6 ICV; exercise plus vehicle; exercise plus ABIL6 (IL-6 antibody); B6- tub/tub : exercise plus vehicle. (E) Evaluation of the cumulative food intake (g) at 4 h, 12 h, and 24 h; (H) AgRP and (I) POMC mRNA expression in the arcuate nucleus of the hypothalamus. Western blot showing (F) TUB tyrosine phosphorylation and (G) TUB associated with <t>JAK2</t> from hypothalamus lysates. The exercise was swimming. All mice were male. ICV: intracerebroventricular. All values are expressed as means ± standard deviation (SD). (A–C) : Sed ( n = 7) and Exe ( n = 6). (D,F,G) : ( n = 4 each group). (E,H,I) : ( n = 5 each group). Unpaired two-tailed t -tests were used to analyze (A–D) ; two-way ANOVA was used to analyze (E) ; one-way ANOVA with Tukey’s multiple comparisons tests was used to analyze (F–I) . * p < 0.0001 vs. other groups; & p < 0.05 vs. other groups; # [Sed + IL-6 vs. Sed + Veh ( p = 0.0411); Sed + Veh vs. Exe + Veh ( p = 0.0002); Sed + IL-6 vs. Exe (B6- tub/tub ) ( p = 0.0314); Exe + Veh vs. Exe (ABIL6) ( p = 0.0004); Exe + Veh vs. Exe (B6- tub/tub ) ( p < 0.0001)].
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Image Search Results


ARMS and α-syntrophin enhanced the EphA4-induced Jak/Stat activation. (A) COS7 cells were transfected with EphA4, EphA4 KD mutant, or ARMS, and cell lysate was immunoblotted with antibodies against phosphorylated Tyr 1007/1008 of mouse Jak2, Tyr 1054/1055 of human Tyk2, Tyr 701 of Stat1, and Thr 202 /Tyr 204 of ERK1/2. p-Tyr; phosphorylated Tyr. (B) Quantification of the Western blots. y axis represents the fold change of tyrosine phosphorylation. n = 3; *, P < 0.05; **, P < 0.005. (C) COS7 cells were transfected with EphA4, ARMS, α-syntrophin, or α-syntrophin mPDZ, and cell lysate was immunoblotted with antibodies against phosphorylated Tyr 1007/1008 of mouse Jak2, Tyr 1054/1055 of human Tyk2, Tyr 701 of Stat1, and Thr 202 /Tyr 204 of ERK1/2. (D) Quantification of the Western blots. n = 3; *, P < 0.05; **, P < 0.005. Error bars represent the SEM.

Journal: The Journal of Cell Biology

Article Title: α-Syntrophin regulates ARMS localization at the neuromuscular junction and enhances EphA4 signaling in an ARMS-dependent manner

doi: 10.1083/jcb.200412008

Figure Lengend Snippet: ARMS and α-syntrophin enhanced the EphA4-induced Jak/Stat activation. (A) COS7 cells were transfected with EphA4, EphA4 KD mutant, or ARMS, and cell lysate was immunoblotted with antibodies against phosphorylated Tyr 1007/1008 of mouse Jak2, Tyr 1054/1055 of human Tyk2, Tyr 701 of Stat1, and Thr 202 /Tyr 204 of ERK1/2. p-Tyr; phosphorylated Tyr. (B) Quantification of the Western blots. y axis represents the fold change of tyrosine phosphorylation. n = 3; *, P < 0.05; **, P < 0.005. (C) COS7 cells were transfected with EphA4, ARMS, α-syntrophin, or α-syntrophin mPDZ, and cell lysate was immunoblotted with antibodies against phosphorylated Tyr 1007/1008 of mouse Jak2, Tyr 1054/1055 of human Tyk2, Tyr 701 of Stat1, and Thr 202 /Tyr 204 of ERK1/2. (D) Quantification of the Western blots. n = 3; *, P < 0.05; **, P < 0.005. Error bars represent the SEM.

Article Snippet: Polyclonal anti-HA antibodies, EphA4 antibody (sc-921), and Jak2 antibody (sc-278) were purchased from Santa Cruz Biotechnology, Inc., and the mAb that recognizes the α-isoform of Stat1 was obtained from Zymed Laboratories.

Techniques: Activation Assay, Transfection, Mutagenesis, Western Blot, Phospho-proteomics

Effects of acute exercise on metabolic parameters and TUB regulation. (A) Body weight (g); (B) 24 h of food intake (g); (C) serum IL-6 levels (pg/ml); and (D) Western blot from hypothalamic lysates showing IL-6 expression in C57BL/6J mice (12–15 weeks of age) under resting conditions (Sed) or after swimming exercise (Exe). B6- tub/+ or B6- tub/tub mice (6–8 weeks of age) were divided into the following groups: B6- tub/+ : sedentary plus vehicle; sedentary plus IL-6 ICV; exercise plus vehicle; exercise plus ABIL6 (IL-6 antibody); B6- tub/tub : exercise plus vehicle. (E) Evaluation of the cumulative food intake (g) at 4 h, 12 h, and 24 h; (H) AgRP and (I) POMC mRNA expression in the arcuate nucleus of the hypothalamus. Western blot showing (F) TUB tyrosine phosphorylation and (G) TUB associated with JAK2 from hypothalamus lysates. The exercise was swimming. All mice were male. ICV: intracerebroventricular. All values are expressed as means ± standard deviation (SD). (A–C) : Sed ( n = 7) and Exe ( n = 6). (D,F,G) : ( n = 4 each group). (E,H,I) : ( n = 5 each group). Unpaired two-tailed t -tests were used to analyze (A–D) ; two-way ANOVA was used to analyze (E) ; one-way ANOVA with Tukey’s multiple comparisons tests was used to analyze (F–I) . * p < 0.0001 vs. other groups; & p < 0.05 vs. other groups; # [Sed + IL-6 vs. Sed + Veh ( p = 0.0411); Sed + Veh vs. Exe + Veh ( p = 0.0002); Sed + IL-6 vs. Exe (B6- tub/tub ) ( p = 0.0314); Exe + Veh vs. Exe (ABIL6) ( p = 0.0004); Exe + Veh vs. Exe (B6- tub/tub ) ( p < 0.0001)].

Journal: Frontiers in Physiology

Article Title: Acute exercise reduces feeding by activating IL-6/Tubby axis in the mouse hypothalamus

doi: 10.3389/fphys.2022.956116

Figure Lengend Snippet: Effects of acute exercise on metabolic parameters and TUB regulation. (A) Body weight (g); (B) 24 h of food intake (g); (C) serum IL-6 levels (pg/ml); and (D) Western blot from hypothalamic lysates showing IL-6 expression in C57BL/6J mice (12–15 weeks of age) under resting conditions (Sed) or after swimming exercise (Exe). B6- tub/+ or B6- tub/tub mice (6–8 weeks of age) were divided into the following groups: B6- tub/+ : sedentary plus vehicle; sedentary plus IL-6 ICV; exercise plus vehicle; exercise plus ABIL6 (IL-6 antibody); B6- tub/tub : exercise plus vehicle. (E) Evaluation of the cumulative food intake (g) at 4 h, 12 h, and 24 h; (H) AgRP and (I) POMC mRNA expression in the arcuate nucleus of the hypothalamus. Western blot showing (F) TUB tyrosine phosphorylation and (G) TUB associated with JAK2 from hypothalamus lysates. The exercise was swimming. All mice were male. ICV: intracerebroventricular. All values are expressed as means ± standard deviation (SD). (A–C) : Sed ( n = 7) and Exe ( n = 6). (D,F,G) : ( n = 4 each group). (E,H,I) : ( n = 5 each group). Unpaired two-tailed t -tests were used to analyze (A–D) ; two-way ANOVA was used to analyze (E) ; one-way ANOVA with Tukey’s multiple comparisons tests was used to analyze (F–I) . * p < 0.0001 vs. other groups; & p < 0.05 vs. other groups; # [Sed + IL-6 vs. Sed + Veh ( p = 0.0411); Sed + Veh vs. Exe + Veh ( p = 0.0002); Sed + IL-6 vs. Exe (B6- tub/tub ) ( p = 0.0314); Exe + Veh vs. Exe (ABIL6) ( p = 0.0004); Exe + Veh vs. Exe (B6- tub/tub ) ( p < 0.0001)].

Article Snippet: The following antibodies were also used and previously validated and published ( ; ; ): p-Tyr (PY20) antibody (mouse monoclonal; Santa Cruz Biotechnology cat# sc-508, RRID:AB_628122), JAK2 (HR-758) antibody (rabbit polyclonal; Santa Cruz Biotechnology cat# sc-278, RRID: AB_631853), IL-6 (H-183) antibody (rabbit polyclonal; Santa Cruz Biotechnology cat# sc-7920, RRID: AB_2127745), and beta-tubulin antibody (rabbit polyclonal; Cell Signaling Technology cat# 2146, RRID: AB_2210545).

Techniques: Western Blot, Expressing, Phospho-proteomics, Standard Deviation, Two Tailed Test

Leptin has no additive effect on food intake and TUB signaling in exercised mice or mice treated with IL-6. (A) Evaluation of cumulative food intake (g) at 4 h, 12 h, and 24 h; Western blot showing (B) TUB tyrosine phosphorylation and (C) TUB associated with JAK2 from hypothalamus lysates from C57BL/6J mice (11–12 weeks of age) under resting conditions (Sed) or after swimming exercise (Exe) treated with vehicle or leptin via intracerebroventricular (ICV). In another independent experiment, we also used C57BL/6J mice (11–12 weeks of age) only under resting conditions (Sed) treated with vehicle or IL-6 and leptin via intracerebroventricular (ICV). We analyzed (D) cumulative food intake (g) at 4 h, 12 h, and 24 h; Western blot showing (E) TUB tyrosine phosphorylation and (F) TUB associated with JAK2 from hypothalamus lysates. We used male mice. All values are expressed as means ± standard deviation (SD). Western blot assays ( n = 4 in each group). Two-way ANOVA was used to analyze (A,D) , while one-way ANOVA with Tukey’s multiple comparisons tests was used to analyze (B,C). * Sed Veh vs. Exe Veh, Sed leptin, and Exe leptin ( p < 0.0011); # Sed Veh vs. Exe Veh, Sed leptin, and Exe leptin ( p < 0.0024); & Sed Veh vs. Exe Veh, Sed leptin, and Exe leptin ( p < 0.0012); ϴ Sed Veh vs. IL-6 Veh, Sed leptin, and IL-6 leptin ( p < 0.0017); § Sed Veh vs. IL-6 Veh, Sed leptin, and IL-6 leptin ( p < 0.0001); ϕp<0.0001 vs. other groups.

Journal: Frontiers in Physiology

Article Title: Acute exercise reduces feeding by activating IL-6/Tubby axis in the mouse hypothalamus

doi: 10.3389/fphys.2022.956116

Figure Lengend Snippet: Leptin has no additive effect on food intake and TUB signaling in exercised mice or mice treated with IL-6. (A) Evaluation of cumulative food intake (g) at 4 h, 12 h, and 24 h; Western blot showing (B) TUB tyrosine phosphorylation and (C) TUB associated with JAK2 from hypothalamus lysates from C57BL/6J mice (11–12 weeks of age) under resting conditions (Sed) or after swimming exercise (Exe) treated with vehicle or leptin via intracerebroventricular (ICV). In another independent experiment, we also used C57BL/6J mice (11–12 weeks of age) only under resting conditions (Sed) treated with vehicle or IL-6 and leptin via intracerebroventricular (ICV). We analyzed (D) cumulative food intake (g) at 4 h, 12 h, and 24 h; Western blot showing (E) TUB tyrosine phosphorylation and (F) TUB associated with JAK2 from hypothalamus lysates. We used male mice. All values are expressed as means ± standard deviation (SD). Western blot assays ( n = 4 in each group). Two-way ANOVA was used to analyze (A,D) , while one-way ANOVA with Tukey’s multiple comparisons tests was used to analyze (B,C). * Sed Veh vs. Exe Veh, Sed leptin, and Exe leptin ( p < 0.0011); # Sed Veh vs. Exe Veh, Sed leptin, and Exe leptin ( p < 0.0024); & Sed Veh vs. Exe Veh, Sed leptin, and Exe leptin ( p < 0.0012); ϴ Sed Veh vs. IL-6 Veh, Sed leptin, and IL-6 leptin ( p < 0.0017); § Sed Veh vs. IL-6 Veh, Sed leptin, and IL-6 leptin ( p < 0.0001); ϕp<0.0001 vs. other groups.

Article Snippet: The following antibodies were also used and previously validated and published ( ; ; ): p-Tyr (PY20) antibody (mouse monoclonal; Santa Cruz Biotechnology cat# sc-508, RRID:AB_628122), JAK2 (HR-758) antibody (rabbit polyclonal; Santa Cruz Biotechnology cat# sc-278, RRID: AB_631853), IL-6 (H-183) antibody (rabbit polyclonal; Santa Cruz Biotechnology cat# sc-7920, RRID: AB_2127745), and beta-tubulin antibody (rabbit polyclonal; Cell Signaling Technology cat# 2146, RRID: AB_2210545).

Techniques: Western Blot, Phospho-proteomics, Standard Deviation

Effects of IL-6 on food intake and TUB regulation. (A) Evaluation of cumulative food intake (g) at 4 h, 12 h, and 24 h ( n = 5 each group); Western blot showing (B) TUB tyrosine phosphorylation from hypothalamus lysates from young (6–8 weeks of age) heterozygous (B6- tub/+ ) or homozygous (B6- tub/tub ) Tubby mice with vehicle or IL-6 ICV injection. (C) 12 h of cumulative food intake (g) ( n = 5 each group); Western blot showing (D) TUB tyrosine phosphorylation, and (E) TUB associated with JAK2 from hypothalamus lysates from lean C3H/Hepas or C3H/HeJ mice under resting conditions (Sed) or after swimming exercise (Exe). (F) 12 h of cumulative food intake (g) ( n = 5 each group); Western blot showing (G) TUB tyrosine phosphorylation, and (H) TUB associated with JAK2 from hypothalamus lysates from lean C3H/Hepas or C3H/HeJ mice treated with vehicle or IL-6 ICV injection. ICV: intracerebroventricular. We used only male mice. All values are expressed as means ± standard deviation (SD). Western blot assays ( n = 3–5 in each group). Two-way ANOVA with Tukey’s multiple comparisons test was used for analyzing all data. (A) & B6- tub/+ plus Veh vs. B6- tub/+ plus IL-6 ( p = 0.0007); B6- tub/+ plus IL-6 vs. B6- tub/tub plus IL-6 ( p = 0.0012); § B6- tub/+ plus Veh vs. B6- tub/+ plus IL-6 ( p < 0.0068); B6- tub/+ plus IL-6 vs. B6- tub/tub plus Veh or IL-6 ( p < 0.0125). (B) *IL-6 increases TUB phosphorylation independently of genotypes ( p = 0.0006); # B6- tub/+ plus IL-6 vs. B6- tub/tub plus IL-6 ( p = 0.0370). (C) , exercise in the Hepas group decreases food intake compared to other groups ( p < 0.0001). (D,E) exercise in the Hepas group increases TUB phosphorylation and association with JAK2 compared to other groups ( p < 0.0001). (F) IL-6 decreases food intake independently of the genotype compared to sedentary groups ( p < 0.0001). (G,H) IL-6 in the Hepas and Hej groups increases TUB phosphorylation and association with JAK2 compared to the sedentary groups ( p < 0.0001).

Journal: Frontiers in Physiology

Article Title: Acute exercise reduces feeding by activating IL-6/Tubby axis in the mouse hypothalamus

doi: 10.3389/fphys.2022.956116

Figure Lengend Snippet: Effects of IL-6 on food intake and TUB regulation. (A) Evaluation of cumulative food intake (g) at 4 h, 12 h, and 24 h ( n = 5 each group); Western blot showing (B) TUB tyrosine phosphorylation from hypothalamus lysates from young (6–8 weeks of age) heterozygous (B6- tub/+ ) or homozygous (B6- tub/tub ) Tubby mice with vehicle or IL-6 ICV injection. (C) 12 h of cumulative food intake (g) ( n = 5 each group); Western blot showing (D) TUB tyrosine phosphorylation, and (E) TUB associated with JAK2 from hypothalamus lysates from lean C3H/Hepas or C3H/HeJ mice under resting conditions (Sed) or after swimming exercise (Exe). (F) 12 h of cumulative food intake (g) ( n = 5 each group); Western blot showing (G) TUB tyrosine phosphorylation, and (H) TUB associated with JAK2 from hypothalamus lysates from lean C3H/Hepas or C3H/HeJ mice treated with vehicle or IL-6 ICV injection. ICV: intracerebroventricular. We used only male mice. All values are expressed as means ± standard deviation (SD). Western blot assays ( n = 3–5 in each group). Two-way ANOVA with Tukey’s multiple comparisons test was used for analyzing all data. (A) & B6- tub/+ plus Veh vs. B6- tub/+ plus IL-6 ( p = 0.0007); B6- tub/+ plus IL-6 vs. B6- tub/tub plus IL-6 ( p = 0.0012); § B6- tub/+ plus Veh vs. B6- tub/+ plus IL-6 ( p < 0.0068); B6- tub/+ plus IL-6 vs. B6- tub/tub plus Veh or IL-6 ( p < 0.0125). (B) *IL-6 increases TUB phosphorylation independently of genotypes ( p = 0.0006); # B6- tub/+ plus IL-6 vs. B6- tub/tub plus IL-6 ( p = 0.0370). (C) , exercise in the Hepas group decreases food intake compared to other groups ( p < 0.0001). (D,E) exercise in the Hepas group increases TUB phosphorylation and association with JAK2 compared to other groups ( p < 0.0001). (F) IL-6 decreases food intake independently of the genotype compared to sedentary groups ( p < 0.0001). (G,H) IL-6 in the Hepas and Hej groups increases TUB phosphorylation and association with JAK2 compared to the sedentary groups ( p < 0.0001).

Article Snippet: The following antibodies were also used and previously validated and published ( ; ; ): p-Tyr (PY20) antibody (mouse monoclonal; Santa Cruz Biotechnology cat# sc-508, RRID:AB_628122), JAK2 (HR-758) antibody (rabbit polyclonal; Santa Cruz Biotechnology cat# sc-278, RRID: AB_631853), IL-6 (H-183) antibody (rabbit polyclonal; Santa Cruz Biotechnology cat# sc-7920, RRID: AB_2127745), and beta-tubulin antibody (rabbit polyclonal; Cell Signaling Technology cat# 2146, RRID: AB_2210545).

Techniques: Western Blot, Phospho-proteomics, Injection, Standard Deviation

Graphical representation of exercise/IL-6 via the JAK2/TUB axis in the hypothalamus. Acute exercise (swimming) or IL-6 treatment ICV induces TUB tyrosine phosphorylation and its association with JAK2 in the hypothalamus of mice. The activation of the IL-6/Tubby axis reduces food intake. Therefore, acute exercise or IL-6 via the activation of the IL-6/JAK2/Tubby axis reduces feeding.

Journal: Frontiers in Physiology

Article Title: Acute exercise reduces feeding by activating IL-6/Tubby axis in the mouse hypothalamus

doi: 10.3389/fphys.2022.956116

Figure Lengend Snippet: Graphical representation of exercise/IL-6 via the JAK2/TUB axis in the hypothalamus. Acute exercise (swimming) or IL-6 treatment ICV induces TUB tyrosine phosphorylation and its association with JAK2 in the hypothalamus of mice. The activation of the IL-6/Tubby axis reduces food intake. Therefore, acute exercise or IL-6 via the activation of the IL-6/JAK2/Tubby axis reduces feeding.

Article Snippet: The following antibodies were also used and previously validated and published ( ; ; ): p-Tyr (PY20) antibody (mouse monoclonal; Santa Cruz Biotechnology cat# sc-508, RRID:AB_628122), JAK2 (HR-758) antibody (rabbit polyclonal; Santa Cruz Biotechnology cat# sc-278, RRID: AB_631853), IL-6 (H-183) antibody (rabbit polyclonal; Santa Cruz Biotechnology cat# sc-7920, RRID: AB_2127745), and beta-tubulin antibody (rabbit polyclonal; Cell Signaling Technology cat# 2146, RRID: AB_2210545).

Techniques: Phospho-proteomics, Activation Assay

Hypothalamic IL-6 action through JAK2 to activate TUB. (A) Evaluation of cumulative food intake (g) at 4 h, 12 h, and 24 h ( n = 5 in each group); Western blot showing (B) TUB tyrosine phosphorylation and (C) TUB associated with JAK2 from hypothalamic lysates from wt/wt mice (6–8 weeks old) obtained in the colony of B6- tub/tub mice under resting conditions (Sed). The mice were treated with vehicle or AG490 (JAK2inhibitor), IL-6, or IL-6 with AG490 by intracerebroventricular (ICV) injections. We used male mice. All values are expressed as means ± standard deviation (SD). Western blot assays ( n = 4 in each group). Two-way ANOVA was used to analyze A, while one-way ANOVA with Tukey’s multiple comparisons tests was used to analyze B and C. *ICV IL-6 vs. Veh, AG490, and IL-6 plus AG490 ( p < 0.0078); # ICV IL-6 vs. Veh, AG490 and IL-6 plus AG490 ( p < 0.0041); ϕ ICV IL-6 vs. Veh, AG490, and IL-6 plus AG490 ( p < 0.0136).

Journal: Frontiers in Physiology

Article Title: Acute exercise reduces feeding by activating IL-6/Tubby axis in the mouse hypothalamus

doi: 10.3389/fphys.2022.956116

Figure Lengend Snippet: Hypothalamic IL-6 action through JAK2 to activate TUB. (A) Evaluation of cumulative food intake (g) at 4 h, 12 h, and 24 h ( n = 5 in each group); Western blot showing (B) TUB tyrosine phosphorylation and (C) TUB associated with JAK2 from hypothalamic lysates from wt/wt mice (6–8 weeks old) obtained in the colony of B6- tub/tub mice under resting conditions (Sed). The mice were treated with vehicle or AG490 (JAK2inhibitor), IL-6, or IL-6 with AG490 by intracerebroventricular (ICV) injections. We used male mice. All values are expressed as means ± standard deviation (SD). Western blot assays ( n = 4 in each group). Two-way ANOVA was used to analyze A, while one-way ANOVA with Tukey’s multiple comparisons tests was used to analyze B and C. *ICV IL-6 vs. Veh, AG490, and IL-6 plus AG490 ( p < 0.0078); # ICV IL-6 vs. Veh, AG490 and IL-6 plus AG490 ( p < 0.0041); ϕ ICV IL-6 vs. Veh, AG490, and IL-6 plus AG490 ( p < 0.0136).

Article Snippet: The following antibodies were also used and previously validated and published ( ; ; ): p-Tyr (PY20) antibody (mouse monoclonal; Santa Cruz Biotechnology cat# sc-508, RRID:AB_628122), JAK2 (HR-758) antibody (rabbit polyclonal; Santa Cruz Biotechnology cat# sc-278, RRID: AB_631853), IL-6 (H-183) antibody (rabbit polyclonal; Santa Cruz Biotechnology cat# sc-7920, RRID: AB_2127745), and beta-tubulin antibody (rabbit polyclonal; Cell Signaling Technology cat# 2146, RRID: AB_2210545).

Techniques: Western Blot, Phospho-proteomics, Standard Deviation

Effects of TUB knockdown on food intake in response to IL-6 ICV injection. (A) Evaluation of food intake (g) at 4 h, 12 h, and 24 h in lean mice under resting conditions treated ICV with sense; IL-6+ASO; ASO; IL-6+sense ( n = 5 each group). Representative quantification of (B) TUB tyrosine phosphorylation and (C) TUB associated with JAK2 from hypothalamus lysates from lean mice under resting conditions treated with IL-6 ICV or vehicle and pre-treated with ASO against TUB or sense. For this experiment, we used male wt/wt mice (6–8 weeks old) obtained from the B6-tub/tub colony. All values are expressed as means ± standard deviation (SD). Western blot assays ( n = 3–4 in each group). Two-way ANOVA was used to analyze A, and one-way ANOVA with Tukey’s multiple comparisons tests was used to analyze B and C. * indicates IL-6 vs. Sense, ASO, and ASO plus IL-6 ( p < 0.0303); # indicates IL-6 vs. Sense, ASO, and ASO plus IL-6 ( p < 0.0042); § indicates Sense vs. ASO, and ASO plus IL-6 ( p <0.0198); & indicates IL-6 vs. Sense, ASO,and ASO plus IL-6 ( p < 0.0092); ϴ indicates Sense vs. ASO, and ASO plus IL-6 ( p < 0.0400).

Journal: Frontiers in Physiology

Article Title: Acute exercise reduces feeding by activating IL-6/Tubby axis in the mouse hypothalamus

doi: 10.3389/fphys.2022.956116

Figure Lengend Snippet: Effects of TUB knockdown on food intake in response to IL-6 ICV injection. (A) Evaluation of food intake (g) at 4 h, 12 h, and 24 h in lean mice under resting conditions treated ICV with sense; IL-6+ASO; ASO; IL-6+sense ( n = 5 each group). Representative quantification of (B) TUB tyrosine phosphorylation and (C) TUB associated with JAK2 from hypothalamus lysates from lean mice under resting conditions treated with IL-6 ICV or vehicle and pre-treated with ASO against TUB or sense. For this experiment, we used male wt/wt mice (6–8 weeks old) obtained from the B6-tub/tub colony. All values are expressed as means ± standard deviation (SD). Western blot assays ( n = 3–4 in each group). Two-way ANOVA was used to analyze A, and one-way ANOVA with Tukey’s multiple comparisons tests was used to analyze B and C. * indicates IL-6 vs. Sense, ASO, and ASO plus IL-6 ( p < 0.0303); # indicates IL-6 vs. Sense, ASO, and ASO plus IL-6 ( p < 0.0042); § indicates Sense vs. ASO, and ASO plus IL-6 ( p <0.0198); & indicates IL-6 vs. Sense, ASO,and ASO plus IL-6 ( p < 0.0092); ϴ indicates Sense vs. ASO, and ASO plus IL-6 ( p < 0.0400).

Article Snippet: The following antibodies were also used and previously validated and published ( ; ; ): p-Tyr (PY20) antibody (mouse monoclonal; Santa Cruz Biotechnology cat# sc-508, RRID:AB_628122), JAK2 (HR-758) antibody (rabbit polyclonal; Santa Cruz Biotechnology cat# sc-278, RRID: AB_631853), IL-6 (H-183) antibody (rabbit polyclonal; Santa Cruz Biotechnology cat# sc-7920, RRID: AB_2127745), and beta-tubulin antibody (rabbit polyclonal; Cell Signaling Technology cat# 2146, RRID: AB_2210545).

Techniques: Knockdown, Injection, Phospho-proteomics, Standard Deviation, Western Blot